How are antibodies used in elisa made?

Welcome to this witty article about “How are antibodies used in ELISA made?” If you’re here, looking for insightful knowledge and a good laugh, then guess what? You’ve just hit the jackpot! Buckle up as we go on a rib-cracking journey with science.

Before We Begin…

But before we begin…I hope you know that it takes a genius to make everything so simple. So, brace yourself because what comes next will knock off your socks!

What is ELISA?

The Enzyme-linked immunosorbent assay (ELISA) is an important technique used to detect infectious diseases and viral infections by measuring the concentration of proteins or antigens within blood serum samples.

Now…let’s get down to business!

Making our Way into It

To create magic antibodies that work seamlessly, we have three potential methods; Mouse hybridoma technology, Phage display library, and Recombinant DNA technology.

Mmmm…Hybridomas

First things first; let us talk about mouse hybridoma technology- my all-time favourite!

This technique involves injecting mice with unknown antigens which trigger their immune system cells (B cells) to respond in defence by producing extensive amounts of various specific antibody molecules against such factors.

From thereon out… It gets tricky: The B cells from these mice would be collected via sampling procedures carried out under strict animal care guidelines. The splenic cells must then combine with immune-effector cells known as “myeloma” – This results in fusion products called “hybridomas.”

Hybridomas consist of two distinct components: One part originates from antigen-reactive B-cells responsible for manufacturing exquisite amounts of high-affinity monoclonal antibody production against such pathogenic agents. Meanwhile, the other component stems from gently malignant tumour-derived model organisms carrying genetic mutations that enable the cells to grow indefinitely in vitro.

Let’s Phage

Secondly, we can’t forget about phage display library technology. Trust me; this is not when I throw a book on a virus!

This method involves manipulating bacteriophage genetic material via gene fusing procedures to generate a collection of various cloned protein fragments (antibodies) showcasing affinity and specificity against different antigens.

These antigen-specific antibody clones are selected by making use of an immobilized target – Viola! This assures the ready isolation of highly specific antibodies with unparalleled sensitivity.

The Third wheel: Recombinant Technology

Finally, what would this article be without mentioning recombinant DNA technology? We don’t want anyone feeling left out!

Also known as “phagemids” for purposes relating both to plasmid vectors alongside lytic phases of bacterial culture selection criteria during cell growth processes- recombinants’ DNAs play critical roles in engineered cellular systems responsible for phenotypic expression activated within E.coli host organisms already present in laboratory environments widely applied across scientific fields concerning cloning and production technologies such as ELISA-assay oriented applications using single-chain variable fragment antibodies or whole IgG molecules/fragments produced from hybridomas undergone further processing manipulations like subcloning steps optimization protocols designed specifically towards increasing yields thereby allowing us have even more precise results. A drastic revolution indeed!

Phew! That was a mouthful. But seriously though, who needs drama series when science endows us such great entertainment?

In conclusion…It takes rigorous approaches — combined with factors favouring excellence — creating magical Antibodies used in ensuring our well-being through several medical analyses such as ELISAs while consistently hitting high-sensitive thresholds capable enough of detecting these infectious diseases before they become too severe resulting in critical diagnostic tools available for medical experts worldwide enabling proficient diagnosis/treatment decisions made possible due to specificity of antibody molecules- precision is key when it comes to medical analysis!

Anyway, I hope you have learned and laughed something new today. Farewell, my friends—they don’t call me the science comedian for nothing!

Thank God For Acronyms!

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